Research
Our current research focus on the role of auxins during the induction of somatic embryogenesis (SE). Most of the SE process requires the presence, either before or during the embryogenic process, of at least one exogenous auxin. This exogenous auxin induces the presence of endogenous auxins, which appears to be essential for SE induction. We are studying the presence of endogenous auxins and its conjugates during the induction of the SE in Coffea spp., Arabidopsis thaliana and Daucus carota, as well as the expression of some genes involved in their homeostasis.
We are also interested in the other component of the homeostasis of the auxins, transport. We are studying the role of some transporters in the mobilization of the auxins and its conjugates during the SE induction.
On the other side, we are studying the role in the SE of the molecules secreted by the explants of Coffea arabica into the culture medium, during the induction of SE. Some of these molecules are able to inhibit the SE process. Whereas some of the proteins secreted into the medium are able to increase the SE. We are elaborating the secretome and the proteome of the spent medium.